
Here are some examples of plants in which senescence has
gone wrong because one or more genes have become
altered.
Genetic interference with senescence often results in
plants which turn yellow abnormally slowly, or late, or
not at all. Such plants are sometimes called
staygreen.
Staygreen is a useful trait in a number of
agricultural and horticultural species, either because
it is associated with improved yield or quality, or
because the appearance of staygreen plants appeals to
consumers.
A. Flageolet beans.
The seeds and pods of this variety do not go yellow like
those of normal (wildtype) beans. Leaves of
flageolet beans also remain green when wildtype leaves
go yellow. This tells us that the same gene
controlling yellowing is normally active in all green
tissues of the plant, and has been inactivated in
flageolet.
B. Wildtype and staygreen lines
of soybean. Both pigment loss and the
shedding of leaves are delayed in the staygreen type.
Quite a few different staygreen genes are known in
soybean, affecting seeds, pods and leaves in different
ways.
C. Staygreen and wildtype
sorghum. Staygreen is an important trait in
sorghum agriculture, associated with improved yield,
drought tolerance and disease resistance in this key
crop of developing countries in the dry tropics.
D. Staygreen and wildtype
maize. Extended greenness of foliage has
been one of the characters of advanced maize varieties
contributing to the huge increase in yields achieved
over the last century or so. Several different
types of staygreen are known. The illustration is
of an experimentally-created transgenic line that overproduces an
anti-senescence hormone.
Leaves of this line pass directly from green to dead
without going through an intervening senescence period.
E. Green and yellow peas.
These have an honoured position in the history of
biology because seed colour was one of the seven
characters studied by Gregor Mendel (that's his
portrait), the father of genetics. Just like flageolet bean (which probably
has the
bean version of Mendel's green pea gene), leaf colour
follows seed colour. Incidentally, the picture on
this page was
taken in Mendel's garden at the Abbey in Brno.
F. Tomato.
Ripening of fruits that start off green and go through a
yellowing process (such as banana) followed by
development of attractive orange and red colours
(tomato, pepper) is essentially a senescence process and
many mutants that block the sequence of events are known
and available at your greengrocer.
G. Staygreen and wildtype
meadow fescue. This senescence mutant was
discovered around 1970 and has kept your humble and
obedient servant busy ever since. We now know
which gene has been altered and how, and found that it
turned out to be the grass equivalent of Mendel's
green/yellow pea gene. Staygreen has been bred
into turf grasses to please gardeners and sports
enthusiasts who like their lawns to be green at all
times.
H. Arabidopsis is the
model species used for plant molecular biology.
Here it has been made into a staygreen by silencing the
equivalent of the grass/Mendel gene. Many other
senescence mutants have been discovered and generated by
scientists working on Arabidopsis.
You can read more about senescence mutants here:
H Thomas, C M Smart (1993) Crops that stay green.
Annals of Applied Biology 123: 193-219
H Thomas, C J Howarth (2000) Five ways to stay green.
Journal of Experimental Botany 51: 329-337
PRH Robson, IS Donnison, Kan
Wang, B Frame, SEl Pegg, A Thomas, H Thomas (2004) Leaf
senescence is delayed in maize expressing the
Agrobacterium IPT gene under the control of a novel
maize senescence-enhanced promoter. Plant Biotechnology
Journal 2: 101-112
I Armstead, I Donnison, S Aubry,
J Harper, S Hörtensteiner, C James, J Mani, M Moffet, H
Ougham, L Roberts, A Thomas, N Weeden, H Thomas, I King
(2007) Cross-species identification of Mendel's I locus.
Science 315: 73 |